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Rabbit Anti-His tag antibody (bs-0287R)
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说明书:50ul  100ul  200ul
20ul/380.00元
50ul/580.00元
100ul/920.00元
200ul/1600.00元
大包装/询价
产品编号bs-0287R
英文名称His tag
中文名称聚组氨酸抗体标签抗体
别    名HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.  
Specific References  (5)     |     bs-0287R has been referenced in 5 publications.
[IF=2.70] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013).  WB ;  Human.  
[IF=2.70] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012).  
[IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969  WB ;  Virus.  
[IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013).  WB ;  Human.  
[IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126.  IHC-P ;  Mouse.  
规格价格50ul/580元购买    100ul/920元购买    200ul/1600元购买    大包装/询价
说 明 书50ul  100ul  200ul
产品类型标签抗体 
研究领域肿瘤  细胞生物  免疫学  染色质和核信号  信号转导  合成与降解  结合蛋白  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应fusionprotein,HHHHHHtagged
产品应用WB=1:500-2000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量N/AkDa
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原Synthetic peptide: HHHHHH conjugated to KLH: 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

SWISS:
N/A

Gene ID:
N/A

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

标签抗体
蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白

1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。
2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。
3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括:
细菌,杆状病毒和哺乳动物)
4) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌
5) 6xHis标签免疫原性差,重组蛋白可以不必除去标签而直接作为抗原进行免疫



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