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Rabbit Anti-Myc tag antibody (bs-0842R)
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说明书:50ul  100ul  200ul
20ul/380.00元
50ul/580.00元
100ul/920.00元
200ul/1600.00元
大包装/询价

产品编号bs-0842R
英文名称Myc tag
中文名称c-Myc tag标签抗体
别    名avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; C-Myc-Tag; class E basic helix-loop-helix protein 39; MRTL; MYC; Myc Epitope Tag; myc proto-oncogene protein; myc-related translation/localization regulatory factor; oncogene c-Myc; proto-oncogene c-Myc; protooncogene homologous to myelocytomatosis virus; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian);MYC_HUMAN.  
规格价格50ul/580元购买    100ul/920元购买    200ul/1600元购买    大包装/询价
说 明 书50ul  100ul  200ul
产品类型标签抗体 
研究领域肿瘤  免疫学  染色质和核信号  信号转导  细胞周期蛋白  转录调节因子  细胞分化  
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, overexpressed proteins containing Myc epitope tag fused
产品应用WB=1:1000-5000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量49kDa
细胞定位细胞核 
性    状Lyophilized or Liquid
浓    度1mg/ml
免 疫 原KLH conjugated C-EQKLISEEDL (human Proto-oncogene c-Myc): 
亚    型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
Epitope tags are useful for the labeling and detection of proteins usingimmunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with KDM5A and KDM5B. Interacts (when phosphorylated at Thr-58 and Ser-62) with FBXW7. Interacts with PIM2 (By similarity). Interacts with NO66.

Subcellular Location:
Nucleus, nucleoplasm. Nucleus, nucleolus.

Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.

SWISS:
P01106

Gene ID:
4609

Database links:

Entrez Gene: 4609 Human

Entrez Gene: 17869 Mouse

Entrez Gene: 24577 Rat

Omim: 190080 Human

SwissProt: P01106 Human

SwissProt: P01108 Mouse

SwissProt: P09416 Rat

Unigene: 202453 Human

Unigene: 2444 Mouse

Unigene: 12072 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
K562 Cell (Human) Lysate at 40 ug
Primary: Anti-Myc tag (bs-0842R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 45 kD
Sample:
Hela Cell (Human) Lysate at 40 ug
Primary: Anti-Myc tag (bs-0842R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 45 kD
Tissue/cell: human endometrium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C-Myc Polyclonal Antibody, Unconjugated(bs-0842R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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